Hartung-Gorre Verlag
Inh.: Dr.
Renate Gorre D-78465
Konstanz Fon:
+49 (0)7533 97227 Fax: +49 (0)7533 97228 www.hartung-gorre.de
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Series in
MICROSYSTEMS
edited by P. A. Besse,
J. Brugger,
M. Gijs,
R. S. Popovic,
Ph. Renaud
Vol. 28:
Kristopher A.
Pataky
Stencil Lithography and Inkjet Printing as
New Tools for Life Sciences Research
2011. XIV, 172 p.; € 64,00. ISBN
3-86628-395-4
This
thesis manuscript describes the application of micro and nanotechnology to
produce three toolkits for life-sciences research. The first technique
presented is nanostencil lithography for patterning
cellular adhesion sites with the ultimate goal of studying mechanosensitive
gene expression. Nanostencil lithography is a
shadow-mask micro and nanopatterning technique that
was adapted for patterning metal on silicone rubber (PDMS) in the course of
this work. Once a specific material contrast is present on the substrate, the
patterns can be chemically functionalized using highly selective surface
modification techniques. In this work, Au micro and nanopatterns
were rendered cell-adhesive by grafting a thiolated
peptide (presenting an RGD moiety) to their surfaces. The micro and nanopatters were used to study whether geometric
confinement could prevent a mammalian cell’s primary ‘focal contacts’ from
developing into mature ‘focal adhesions’. Au micro and nanopatters
were successfully created on PDMS, glass, and even polytetrafluoroethylene.
The
second part of this manuscript focuses on 3D bioprinting.
Recently, 3D printing has received attention as a possible means of assembling
heterogeneous tissue mimetics and ultimately entire organs. However, to date no
one has shown true 3D printing of hydrogels in a ‘block by block’ manner
analogous to industrial rapid prototyping systems. One of the main hurdles is
the fact that printed hydrogels tend to show complete spreading on other
printed hydrogels (or, like spreads on like). This work details how the
material properties of a hydrogel system can be optimized to obtain 3D hydrogel
printing analogous to a rapid prototyping system. It goes on to show that this
optimized printing process enables the fabrication of branched microvasculature
- a previously undemonstrated but key requirement in the engineering of bulk
tissues and organs.
The final part
of this manuscript describes the creation of an X-ray microcollimator
to study subcellular and subnucleolar damage responses
in cells. While many molecular biologists use ionizing radiation (commonly from
X-ray tubes) to induce damage in cells, current X-ray microcollimators
only work well with costly synchrotron radiation sources. During the course of
this thesis, an X-ray microcollimator was developed
that is compatible with conventional X-ray tube setups. The device collimates
20 - 30 keV X-rays into irradiation stripes between
0.5 and 10 µm in diameter.
Results with the microcollimator
show that it is effective in limiting X-ray damage to single sub-cellular and
sub-nucleolar stripe zones.
Keywords:
BioMEMS, biomaterials, nanopatterning, surface
modification, ionizing radiation, microcollimator, bioprinting, inkjet, hydrogels, tissue engineering, life sciences.
Direkt bestellen bei / to order directly from:
Hartung-Gorre
Verlag / D-78465 Konstanz / Germany
Telefon: +49 (0) 7533 97227 Telefax: +49 (0) 7533 97228
http://www.hartung-gorre.de eMail: verlag@hartung-gorre.de